# Nanofibrous Membranes Based on Collagen and Conductive Polymers with Perspective for Biological Applications

## TL;DR

Researchers created nanofibrous membranes using collagen, chitosan, and conductive polymers, which could be useful for tissue regeneration and electrically active biomedical applications.

## Contribution

The study introduces a novel method to fabricate biocompatible nanofibrous membranes with conductive polymers for tissue engineering.

## Key findings

- C-Ch/Ppy membranes showed better elasticity and stress distribution than C-Ch/Pani membranes.
- C-Ch membranes exhibited higher resistance to Ca2+ ion diffusion compared to Na+.
- H9C2 cells adhered successfully to the membranes, indicating good biocompatibility.

## Abstract

In this study, membranes of collagen–chitosan (C-Ch) in combination with conductive polymers (CPs) such as polyaniline (Pani) and polypyrrole (Ppy) were obtained by electrospinning using non-toxic solvents such as PBS and ethanol. The change in the morphology after swelling was observed by SEM, while an FTIR analysis showed specific interactions between C-Ch and CP. Mechanical tests showed that C-Ch/Ppy exhibited more elastic behavior and a better stress distribution compared to C-Ch/Pani. The diffusion of Na+ and Ca2+ ions through the membranes was evaluated and showed a greater resistance for Ca2+ in both membrane types. Preliminary biocompatibility testing with H9C2 cells showed a successful cell adhesion to the membranes. These results emphasize the potential of C-Ch/Pani composites for electrically active scaffolds and of C-Ch/PPy composites for applications in mechanically dynamic tissue-specific regeneration.