Effect of a Polyhexanide-Based Antiseptic Composition on Dentin Microhardness and Mechanical Properties: An In Vitro Study

TL;DR

This study compares how different antiseptics affect the hardness and strength of dentin, finding that polyhexanide-based solutions are less damaging than common alternatives like sodium hypochlorite.

Contribution

The study introduces polyhexanide-based antiseptics as a potentially safer alternative to traditional root canal irrigants in preserving dentin mechanical properties.

Findings

Sodium hypochlorite (3%) significantly reduced dentin microhardness and mechanical strength.

Polyhexanide-based solutions (0.1% and 0.2% Lavasept) had a milder effect, with only 0.2% showing significant changes in elastic deformation energy.

EDTA caused severe surface destruction, making post-treatment measurements unreliable.

Abstract

The effect of root canal irrigants on the mechanical properties of dentin is crucial in endodontic treatment planning. While antiseptics such as sodium hypochlorite and EDTA are widely used, their potential to weaken dentin structure remains a concern. Polyhexanide-based formulations may offer a safer alternative. To assess the impact of a polyhexanide-based antiseptic composition, compared to standard irrigants, on the microhardness, Young’s modulus, and elastic deformation energy of dentin. Sixty extracted human teeth were sectioned and polished to prepare dentin samples. Baseline measurements of Vickers microhardness, Young’s modulus, and elastic deformation work were performed using a Microhardness Tester (CSM Instruments, Switzerland) with a Berkovich indenter. Samples were then divided into six groups (n = 10 per group) and exposed to different irrigants (NaCl 0.9%, NaOCl 3%, chlorhexidine 2%, EDTA 17%, and polyhexanide-based solutions—0.1% and 0.2% Lavasept). Post-treatment measurements were performed. Statistical analysis was conducted using non-parametric tests with Bonferroni correction. Sodium hypochlorite (3%) caused the most pronounced reduction in dentin microhardness and mechanical strength, though not always statistically significant. Polyhexanide-based solutions (0.1% and 0.2% Lavasept) showed a milder effect, with statistically significant changes observed only in elastic deformation energy for 0.2% polyhexanide. EDTA treatment led to severe surface destruction, precluding reliable post-treatment measurements. Polyhexanide-based irrigants demonstrated a more favorable impact on dentin mechanical properties compared to traditional irrigants, supporting their potential use in endodontic protocols aimed at preserving dentin integrity.

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