The Isolation and Construction of an Infectious Clone for a Duck Adenovirus Type 3 Strain

TL;DR

Researchers created an infectious clone of a duck adenovirus strain, which can help study the virus's biology and develop future treatments.

Contribution

The paper introduces a reverse genetics system for duck adenovirus type 3 using an infectious clone.

Findings

The rescued virus (rSD2019) showed similar growth to the wild-type strain in LMH cells.

rSD2019 exhibited comparable replication and virulence in Muscovy ducks as the original strain.

The reverse genetics system for DAdV-3 was successfully established.

Abstract

A duck adenovirus type 3 strain, SD2019, was isolated from sick Muscovy ducks in our laboratory in 2019. To study the biological properties of the virus, an infectious clone of the SD2019 strain was successfully established. The plasmid containing the whole genome of DAdV-3 was digested with Pac I and the linearized DNAs were electortransfected into LMH cells; the cells showed cytopathic effects (CPEs) at 96 h post transfection and the rescued virus (rSD2019) was identified by PCR and indirect immunofluorescence assays (IFAs). The biological characteristics of strain rSD2019 were studied in vitro and in vivo and the results show that rSD2019 grew to similar titers as compared with the wild-type SD2019 strain in LMH cells, as well as showing similar replication and virulence in Muscovy ducks. The establishment of a reliable reverse genetics system for DAdV-3 provides a foundation for future studies of DAdV-3.

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