DPPC Membrane Under Lateral Compression and Stretching to Extreme Limits: Phase Transitions and Rupture

TL;DR

This study explores how a DPPC membrane behaves under extreme lateral compression and stretching, revealing phase transitions and rupture points.

Contribution

The study provides precise pressure ranges for phase transitions and rupture in DPPC membranes under extreme mechanical stress.

Findings

DPPC membranes undergo a phase transition to an undulated state between 40 and 50 bar under compression.

Membrane rupture becomes certain at −200 bar during stretching.

Microsecond-scale simulations are necessary to capture critical phenomena under extreme stress.

Abstract

Dipalmitoylphosphatidylcholine (DPPC), is one of the key bilayer membranes of the phosphatidylcholine (PC) family which constitutes 40–50% of total cellular phospholipids in mammal cells. We investigate the behavior of an initially planar DPPC membrane under lateral pressures from −200 to 150 bar at 323 K using microsecond-scale simulations. We identify, with very high precision, the pressure range for the occurrence of critical phenomena, mainly undulation and rupture. Notably, under compression, the membrane initially thickens, leading to a phase transition to an undulated state between 40 and 50 bar, as gauged by the sharp changes in the diverse structural metrics. Stretching induces systematic membrane thinning, with rupture becoming probable at −170 bar and certain at −200 bar. The reverse compression cycle shows pressure hysteresis with a 10-bar shift, while the reverse stretching cycle retraces the pathway. System size has a minimal impact on the observed trends. Under extreme mechanical stress, particularly near critical phenomena, simulation times on the order of microsecond are needed to accurately capture phase behavior and structural alterations. This work provides important insights into understanding membrane behavior under extreme conditions, which are relevant to numerous biological and technological applications.

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