# Multiomics Analysis Reveals Role of ncRNA in Hypoxia of Mouse Brain Microvascular Endothelial Cells

## TL;DR

This study explores how non-coding RNAs (ncRNAs) respond to low oxygen in brain blood vessel cells, revealing their role in disease processes like stroke.

## Contribution

The study identifies specific ncRNA responses to hypoxia in brain endothelial cells, offering new insights into blood-brain barrier dysfunction.

## Key findings

- Hypoxia significantly downregulates ncRNA processing pathways in brain microvascular endothelial cells.
- Levels of miRNAs, tRNAs, tsRNAs, snoRNAs, lncRNAs, and circRNAs are upregulated under hypoxia.
- ncRNAs may contribute to oxidative stress and cellular adaptation during hypoxia, impacting blood-brain barrier integrity.

## Abstract

Hypoxia leads to endothelial dysfunction and increased blood–brain barrier (BBB) permeability, promoting the incidence of diseases such as stroke and acute high-altitude illness. Brain microvascular endothelial cells (BMECs) are important structural and functional components of the BBB; however, the molecular changes that occur in BMECs during hypoxia remain unknown. We reported the molecular and functional changes in BMECs under hypoxia through whole-transcriptome sequencing, small RNA microarray, TMT quantitative proteomic, and untargeted metabolomic analyses. We found that hypoxia affected pathways such as ncRNA processing, the HIF-1 signaling pathway, the cell cycle, DNA replication, glucose metabolism, protein synthesis, and inflammation pathways. ncRNA processing was significantly downregulated. However, the levels of some miRNAs, tRNAs, tsRNAs, snoRNAs, lncRNAs, and circRNAs were significantly upregulated under hypoxia. These results suggest that ncRNAs may play an important role in oxidative stress and cellular adaptation to hypoxia, helping us understand the pathological process of BBB injury and providing potential targets for the treatment of BBB-related cerebrovascular diseases.