# Swt21p Is Required for Nam8p-U1 snRNP Association and Efficient Pre-mRNA Splicing in Saccharomyces cerevisiae

## TL;DR

This study shows that the Swt21 protein helps stabilize the U1 snRNP complex, which is important for efficient pre-mRNA splicing in yeast.

## Contribution

The novel finding is that Swt21p is a spliceosome regulator that stabilizes U1 snRNP during early spliceosome assembly.

## Key findings

- Deletion of SWT21 causes intron retention and exon skipping in SUS1 RNA.
- Swt21p is required for the stable association of Nam8p with U1 snRNP.
- Swt21p-associated proteins are involved in cellular processes beyond splicing.

## Abstract

While the U1 small nuclear ribonucleoprotein (snRNP) plays a crucial role in early spliceosome assembly, the mechanisms by which it coordinates with other splicing factors for efficient assembly remain elusive. This study aimed to examine the role of the Swt21 protein in regulating U1 snRNP in Saccharomyces cerevisiae. Swt21p was required for efficient pre-mRNA splicing both in vivo and in vitro. Deletion of SWT21 altered the splicing patterns of two-intron SUS1 RNA, causing intron retention and exon skipping. Spliceosome assembly analysis revealed that in the pre-B complex, the levels of U1 protein components, as well as U1 RNA, were decreased following SWT21 deletion, highlighting the compromised stability of U1 snRNP during this stage. Consistently, in the absence of Swt21p, free isoform of U1 component Nam8p was observed, and its proper nuclear localization was disrupted, demonstrating the functional importance of Swp21p for the stable association of Nam8p with U1 snRNP. Moreover, Swt21p remained primarily in a free state under physiological conditions and did not associate with the pre-B complex. Additionally, TAP analysis revealed that Swt21p-associated proteins are involved in cellular processes beyond splicing. These findings collectively indicate that Swt21p functions as a spliceosome regulator rather than a core component and support a model wherein Swt21p contributes to U1 snRNP stability during early spliceosome assembly.