# Heterologous Expression of Recombinant Ginseng Tetradecapeptide in Saccharomyces cerevisiae and Evaluation of Its Biological Activity

## TL;DR

Scientists expressed a ginseng peptide in yeast and found it improves glucose uptake in fat cells, possibly by affecting metabolism and insulin signaling.

## Contribution

A ginseng tetradecapeptide was heterologously expressed in yeast and shown to enhance glucose uptake in adipocytes via lipid metabolism and insulin signaling modulation.

## Key findings

- 7RS14α significantly increased glucose uptake in high-glucose mouse adipocytes.
- Transcriptomic analysis suggested modulation of lipid metabolism and insulin signaling pathways.
- The peptide may influence energy homeostasis in adipocytes.

## Abstract

Ginseng peptides, as bioactive components of ginseng, have attracted increasing attention. In this study, a 14-amino acid ginseng peptide was selected and heterologously expressed in Saccharomyces cerevisiae using a multicopy tandem fusion strategy, named 7RS14α. The secondary structure of the recombinant ginseng tetradecapeptide (7RS14α) was analyzed, and a high-glucose model was established in mouse adipocytes to evaluate its biological activity. Transcriptomic profiling was further performed to elucidate its potential mechanisms. Results demonstrated that 7RS14α significantly enhanced glucose uptake in high-glucose model cells, likely by modulating lipid metabolism pathways and insulin signaling cascades, thereby influencing energy homeostasis in adipocytes.