# Hepatitis B Virus Genotypes and Subgenotypes Circulating in Belarus

## TL;DR

This study identifies the main hepatitis B virus genotypes in Belarus and finds that local variants are primarily responsible for the ongoing epidemic.

## Contribution

The study provides the first comprehensive analysis of HBV genotypes and subgenotypes circulating in Belarus.

## Key findings

- Genotype D is the most prevalent (81.7%) in Belarusian HBV samples.
- Local variants of the virus are the main drivers of the hepatitis B epidemic in Belarus.
- Occasional introductions of new genetic variants from outside the country have been observed.

## Abstract

Approximately 800–900 new cases of chronic forms of hepatitis B are reported in Belarus annually. Compulsory vaccination, introduced to the country in the mid-90s, produces a certain positive effect on reducing the number of HBV cases. However, around 75,000 patients with chronic hepatitis B are estimated to live in the country at the moment. The main goal of this research was to determine the genotypes/subgenotypes of the hepatitis B virus and establish their role in maintaining the epidemic process in the country. Serological (CMIA, ELISA), molecular biological (PCR, sequencing), and bioinformatic (phylogenetic analysis) methods have been used to obtain results. As studies have shown, in 722 (81.7%) samples, genotype D has been determined; in 156 (17.7%), genotype A; in 3 (0.3%), genotype C; and only in 1 sample (0.1%), genotype B. In two cases (0.2%), a recombinant form of the hepatitis B virus has been detected. The epidemic process of hepatitis B in the country is supported mainly by the circulation of “local” variants of the virus. At the same time, there are occasional introductions of new genetic variants from outside of the country.