# Effect of prevaccination blood and T-cell phenotypes on antibody responses to a COVID-19 mRNA vaccine

## TL;DR

This study identifies pre-vaccination blood and T-cell markers that predict antibody responses to a COVID-19 mRNA vaccine.

## Contribution

The study introduces novel pre-vaccination predictors of IgG responses to a COVID-19 mRNA vaccine.

## Key findings

- Higher frequencies of interferon-γ+ spike-specific CD4+ T cells correlate with stronger antibody responses.
- Lower percentages of naïve CD8+ T cells and lower hemoglobin levels predict lower antibody titers.
- Pre-existing spike-reactive T cells do not influence peak IgG levels.

## Abstract

Despite the high effectiveness of the coronavirus disease 2019 (COVID-19) mRNA vaccines, both immunogenicity and reactogenicity show substantial interindividual variability. One key challenge is predicting high and low responders using easily measurable parameters. In this study, we performed multivariate linear regression analysis, which allows adjustment for confounding, to explore independent predictive factors for antibody responses. Using data from 216 healthy vaccinated donors aged 23–81 years, we evaluated baseline characteristics, prevaccination blood and T-cell phenotypes, and post-vaccination T-cell responses as variables, with anti-receptor-binding domain (RBD) immunoglobulin G (IgG) titers following two doses of BNT162b2 vaccination as the primary outcome. Consistent with previous reports, higher age, a history of allergic disease, and autoimmune disease were associated with lower peak IgG titers. Additionally, the frequencies of interferon-γ+ spike-specific CD4+ T cells (T-cell response) following the first vaccination strongly correlated with higher IgG responses, while those of pre-existing spike-reactive T cells showed no association with peak IgG titers. Furthermore, we identified lower percentages of naïve CD8+ T cells, lower hemoglobin levels, lower lymphocyte counts, and higher mean corpuscular volume as independent pre-vaccination predictors of lower peak IgG levels. Notably, the frequency of naïve CD8+ T cells showed a positive correlation with the peak IgG levels even in univariate analysis. These findings contribute to the individualized prediction of mRNA vaccine efficacy and may provide insights into the mechanisms underlying individual heterogeneity in immune responses.

Novel predictors of IgG responses to a COVID-19 mRNA vaccine