The SlymiR394-ZDS Module Enhances the Antioxidant Capacity of Tomato by Promoting Lycopene Synthesis
Qiuyu Zhao, Li Zhao, Jiuzhi Shi, Xiaoxiao Chen, Zhenchao Yang, Yongjun Wu
TL;DR
This study shows that miR394 regulates lycopene synthesis in tomatoes, boosting their antioxidant capacity through the ZDS gene.
Contribution
The study reveals a novel miR394-ZDS regulatory module involved in lycopene metabolism and antioxidant enhancement in tomatoes.
Findings
Silencing miR394 increased ZDS gene expression and lycopene accumulation in transgenic tomato plants.
Antioxidant enzyme activities and free radical scavenging ability were significantly improved in miR394-silenced plants.
miR394 directly inhibits ZDS expression in chloroplasts, as confirmed by subcellular localization experiments.
Abstract
Lycopene, a natural pigment, is valuable for human health because of its strong antioxidant capacity. However, studies on the involvement of tomato miR394 in the regulation of lycopene have not been reported. The aim of this study was to reveal the molecular mechanism by which miR394 regulates lycopene synthesis by targeting ζ-carotene dehydrogenase (ZDS). The miR394-silenced transgenic tomato plants were constructed by short tandem target mimicry (STTM) technology, and the association between lycopene content and antioxidant capacity was analyzed by combining qRT-PCR, UV spectrophotometry, and a free radical scavenging assay. The targeting relationship between miR394 and ZDS was verified using a subcellular localization assay. The results showed that the silencing of miR394 significantly upregulated the expression of the ZDS gene and promoted lycopene accumulation. The antioxidant…
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Taxonomy
TopicsAntioxidant Activity and Oxidative Stress · Plant Molecular Biology Research · Plant biochemistry and biosynthesis