# GCN5 Is a Master Regulator of Gene Expression in the Malaria Parasite Plasmodium falciparum

## TL;DR

This study shows that PfGCN5 is a key regulator of gene expression in the malaria parasite Plasmodium falciparum, controlling both general and parasite-specific processes.

## Contribution

The study identifies PfGCN5 as a master regulator and uses CUT&Tag-seq to reveal its chromatin localization and interaction with AP2 transcription factors.

## Key findings

- PfGCN5 knockdown caused fewer transcriptional changes than bromodomain deletion.
- CUT&Tag-seq identified more H3K9ac peaks and showed higher sensitivity in mapping PfGCN5 localization.
- PfGCN5 colocalizes with AP2-LT and AP2 motifs, indicating its recruitment by AP2 transcription factors.

## Abstract

GCN5-containing SAGA complex is evolutionarily conserved across yeast, plants, and humans and acts as a general transcription coactivator in the genome-wide regulation of genes. In Plasmodium falciparum, PfGCN5 forms a divergent complex, and the mis-localization of this complex by deleting the PfGCN5 bromodomain (ΔBrd) causes a plethora of growth defects. To directly test the PfGCN5 function, we performed conditional knockdown (KD) of PfGCN5. Whereas PfGCN5 KD phenotypically recapitulated the ΔBrd growth defects, it caused fewer transcriptional alterations compared to ΔBrd. To decipher the mechanism by which PfGCN5 regulates gene expression, we applied a new chromatin landscape analysis tool, CUT&Tag-seq, to map the chromatin localization of PfGCN5 and its deposited histone mark H3K9ac. Compared to ChIP-seq, CUT&Tag-seq identified substantially more H3K9ac peaks in the promoters of its target genes, with the peak intensity positively correlated with the levels of gene expression. CUT&Tag-seq analysis was remarkably more sensitive in mapping chromatin positions of PfGCN5, which colocalized with H3K9ac. The genes enriched with PfGCN5/H3K9ac signals at their promoters are involved in broad biological processes. Notably, PfGCN5′s positions overlapped with sequence motifs recognized by multiple apetela2 (AP2)-domain-containing transcription factors (AP2 TFs), suggesting that they recruited PfGCN5 to these promoters. Additionally, PfGCN5 was also colocalized with AP2-LT, further validating that AP2-LT is an integral component of the PfGCN5 complex. Collectively, these findings establish PfGCN5 as a master gene regulator in controlling general and parasite-specific cellular processes in this low-branching parasitic protist.