# Case of Japanese Marten (Martes melampus) Identification by mtDNA Analysis in a Series of Vehicle Cable Damage Incidents

## TL;DR

This paper describes how mtDNA analysis identified Japanese martens as the cause of vehicle cable damage in Japan, showing how DNA evidence can be used in wildlife-related criminal cases.

## Contribution

The study demonstrates the use of mtDNA analysis in forensic veterinary investigations to identify wildlife responsible for property damage.

## Key findings

- mtDNA analysis confirmed the Japanese marten (Martes melampus) as the species responsible for vehicle cable damage.
- DNA evidence from damaged cables was successfully used to identify the animal species involved in the incidents.
- The findings suggest that mtDNA analysis is a valuable tool in forensic veterinary investigations and ecological research.

## Abstract

In this study, mitochondrial DNA (mtDNA) analysis was performed to identify the animal species involved in an actual case of vehicle component damage that occurred in an urban area of Japan. The results confirmed that such genetic testing can provide scientific evidence demonstrating that damage to man-made objects was caused by animals.

A series of incidents involving damage to vehicle speed sensor cables occurred in an urban area in Japan. At the request of the police, DNA analysis was conducted to identify the animal species responsible. Swab samples collected from the damaged sections of the cables were subjected to PCR testing using mtDNA fragments. Sequencing analysis with universal primers (SCPH02500, SCPL02981) detected DNA from the Japanese marten (Martes melampus). A comprehensive examination that included morphological analysis of the cable damage and consideration of the ecological characteristics of the Japanese martens suggested that the damage was likely caused by this species. DNA analysis using mtDNA markers is a valuable tool for species identification in wildlife forensic veterinary investigations and serves as important scientific evidence in criminal cases involving animals. The findings from this case may contribute to future investigations in forensic veterinary science and ecological research and may also inform measures to prevent human–wildlife conflicts involving animals.