# Leucine-rich pentatricopeptide repeat-containing protein (LRPPRC)-stabilized lncRNA small nucleolar RNA host gene 15 (Snhg15) modulates hematopoietic injury induced by γ-ray irradiation via m6A modification

## TL;DR

This study shows how a specific RNA modification helps worsen radiation damage to blood cells and suggests new ways to treat radiation injuries.

## Contribution

The study identifies LRPPRC as a novel m6A reader that stabilizes Snhg15 lncRNA to modulate radiation-induced hematopoietic injury.

## Key findings

- γ-radiation causes hematopoietic injury by inducing apoptosis, oxidative stress, and DNA damage in BMCs.
- LncRNA Snhg15 is a key regulator of radiation injury progression through m6A modification.
- Knockdown of LRPPRC or Snhg15 reduces radiation-induced hematopoietic damage in mice.

## Abstract

With advancements in radiotherapy technologies, the detrimental effects of ionizing radiation on biological systems, particularly the hematopoietic system, have caused significant concern. N6-methyladenosine (m6A), the most pervasive representative of post-transcriptional modifications, plays critical roles in diverse biological events. Non-coding RNA comprises the vast majority of the human genome. This study aimed to explore the role of long non-coding RNA (lncRNA) m6A modification in γ-ray irradiation-induced hematopoietic injury. By using mouse models, it was found that γ-radiation rapidly damaged hematopoietic bone marrow cells (BMCs), triggering apoptosis, oxidative stress and DNA damage, along with up-regulation of m6A Reader proteins. We revealed the time-conditioned landscape of lncRNA m6A methylome of BMCs in the short term after radiation and found that a dynamic “change-then-recover” trend involved. LncRNA Snhg15 was identified as a key regulator through integration analysis of the methylome and transcriptome data. Its m6A modification was closely related to progression of radiation injury in BMCs. Further research demonstrated that the novel m6A Reader LRPPRC could interact with the modification site of Snhg15, stabilize Snhg15 and promote its expression, thereby exacerbating radiation-induced injury to BMCs both in vitro and in vivo. Knockdown of Lrpprc or Snhg15 could alleviate the radiation injury to the hematopoietic system. Additionally, the LRPPRC-Snhg15 axis was involved in the radio-protective efficacy of gut microbiota-derived valeric acid. These findings uncover a novel mechanism by which m6A-modified lncRNA Snhg15 is stabilized by LRPPRC modulates γ-irradiation-induced hematopoietic injury, providing potential therapeutic targets for the prevention and treatment of radiation injuries.

The online version contains supplementary material available at 10.1186/s43556-025-00279-2.