# Comparative study of the glucose and trehalose addition on the extenders for goat sperm liquid storage

## TL;DR

This study compares the effects of glucose and trehalose on goat sperm quality during cold storage, finding that trehalose performs better after 48 hours.

## Contribution

The study introduces a comparative analysis of glucose and trehalose in semen extenders for goat sperm storage.

## Key findings

- Trehalose at 75 mm showed better sperm motility and integrity after 48 hours of storage.
- Glucose and trehalose at 150 mm reduced sperm quality compared to lower concentrations.
- Trehalose outperformed glucose in preserving sperm motility and functional integrity.

## Abstract

The objective of this study was to assess the effects of varying glucose and trehalose concentrations on tris-citric acid-egg yolk-fructose (TCEF) diluents for the short-term cold storage of goat semen.

The semen sample was collected, unwashed, and divided into the following groups: control (TCEF without glucose and trehalose), TCEF + glucose (75, 150 mm), and TCEF + trehalose (75, 150 mm). Each experimental sperm group (sperm concentration: 9 × ×107/ml) was kept at 4°C in a refrigerator. The impact of varying glucose and trehalose levels on the quality of the spermatozoa was assessed at different time points: after dilution and at 5, 24, 48, and 72 h of refrigeration.

After dilution, progressive motility (PM), total motility (TM), sperm viability (SV), functional integrity (FI), and acrosome integrity of G-75, G-150, T-75, and T-150 did not differ significantly from the control. The PM, TM, SV, FI, and acrosome integrity of sperm of T-150 were considerably lower than the control, G-75, G-150, and T-75 after 5 and 24 h of cool storage. The T-75 group showed superior PM, TM, and FI after 48 h of cool storage, with noticeably greater values than the other groups.

This study indicates that trehalose is a more favorable sugar than glucose for 48 h cool storage of buck semen, providing greater advantages in PM, TM, and PMI.