The genetic mapping and phenotypic analysis of Patronin F.1.1 in Drosophila melanogaster

TL;DR

This study identifies a new mutation in the Patronin gene in fruit flies that causes abnormal eye development.

Contribution

The paper reports a novel allele of the Patronin gene linked to a rough eye phenotype in Drosophila.

Findings

The F.1.1 mutation in Patronin causes a rough eye phenotype due to reduced mutant tissue representation.

Genetic mapping confirmed the mutation's location within the Patronin gene using deficiency crosses.

Sequencing validated the novel allele of the Patronin gene.

Abstract

The multi-institutional Fly-CURE project is an undergraduate genetics research initiative centered on
Drosophila melanogaster
as a model organism. This study aimed to characterize and map mutations discovered through a Flp/FRT EMS screen to investigate complex interactions among genes associated with cell division, growth, and apoptosis leading to abnormal cell proliferation. The
F.1.1
mosaic phenotype resulted in a rough eye phenotype with an overall decrease in representation of mutant tissue. To genetically map the location of the
F.1.1
mutation, flies with genotype

FRT42D,F.1.1,
Dark
82
/CyO

were crossed with the Bloomington 2R Deficiency Kit. The resultant F1 progeny were analyzed to pinpoint mapping deficiencies. The genomic region containing the

Patronin

gene was identified and sequencing confirmed the novel allele of

Patronin
F.1.1

.

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