Inhibition of α-Amylase and α-Glucosidase Using Baccaurea ramiflora

TL;DR

This study explores the antidiabetic potential of Baccaurea ramiflora by testing its extracts for enzyme inhibition linked to diabetes.

Contribution

The study identifies specific B. ramiflora extracts with strong α-amylase and α-glucosidase inhibition for diabetes management.

Findings

Flesh with seed and peel extracts showed the highest enzyme inhibition with low IC50 values.

Leaf extract had the highest flavonoid content, while phenolic content was highest in leaves, flesh with seed, and peel.

GC-MS analysis revealed bioactive compounds like phenol and methyl salicylate in the extracts.

Abstract

The hunt for alternative antidiabetic treatments that result in fewer side effects judged against established treatments is being powered by the mounting worldwide pervasiveness of diabetes mellitus. Playing a critical role in many biological processes, Baccaurea ramiflora, a long-established medicinal plant commonly used throughout Southeast Asia, presents abundant health benefits. An assessment of the various ethanol extracts from B. ramiflora drawn from the leaves, stems, stem bark, peel, and flesh with seed revealed the total phenolic and flavonoid contents in addition to the levels of inhibitory activity against the enzymes α-amylase and α-glucosidase. In the case of total phenolic and flavonoids contents, the findings confirmed that the leaves, flesh with seed, and peel extracts were the richest source of phenolics. The leaf extract showed higher flavonoid content than others. The greatest levels of inhibitory activity against the two enzymes of interest were exhibited by the flesh with seed and peel: the respective IC50 values associated with α-amylase and α-glucosidase were 0.50 ± 0.03 and 0.69 ± 0.01 mg mL−1 for flesh with seed, and 0.61 ± 0.04 and 0.59 ± 0.01 mg mL−1 for peel. The various extracts were all capable of inhibiting enzyme activity in a concentration-dependent manner. GC-MS analysis of the flesh with seed extract identified phenol, 3,5-bis(1,1-dimethylethyl)-, dimethylsulfoxonium formylmethylide, methyl salicylate, and other compounds. Additionally, the major compounds present in the peel extract were 2,4-di-tert-butylphenol, dimethyl sulfone, cyclooctasiloxane, hexadecamethyl, and other compounds. From these findings, B. ramiflora offers potential for use in the field of nutraceuticals and functional foods and might also find application in treating health conditions such as diabetes.

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