# In vitro reconstitution of SPO11‐mediated DNA cleavage reveals mechanistic and clinical insights into meiotic DNA double‐strand break formation

**Authors:** Zetao Hu, Xinzhe Tang, Ming‐Han Tong, Ying Huang

PMC · DOI: 10.1002/ctm2.70383 · 2025-06-22

## Full-text entities

- **Genes:** TOP6BL (TOP6B like initiator of meiotic double strand breaks) [NCBI Gene 79703] {aka C11orf80, HYDM4, TOPOVIBL}, H2ax (H2A.X variant histone) [NCBI Gene 15270] {aka H2A.X, H2afx, Hist5-2ax, gammaH2ax}, Top6bl (TOP6B like initiator of meiotic double strand breaks) [NCBI Gene 381196] {aka Gm960, TOPOVIBL}, Sycp1 (synaptonemal complex protein 1) [NCBI Gene 20957] {aka SCP1}, TDP2 (tyrosyl-DNA phosphodiesterase 2) [NCBI Gene 51567] {aka AD022, EAP2, EAPII, TTRAP, dJ30M3.3, hTDP2}, Rpa2 (replication protein A2) [NCBI Gene 19891] {aka RPA34, Rf-A2}
- **Diseases:** male infertility (MESH:D007248), premature ovarian insufficiency (MESH:D016649), oligospermia (MESH:D009845), aneuploidy (MESH:D000782), synapsis abnormalities (MESH:D000014), Infertility (MESH:D007246)
- **Chemicals:** Cy3 (-)
- **Species:** Mus musculus (house mouse, species) [taxon 10090], Homo sapiens (human, species) [taxon 9606]
- **Mutations:** rs28368082, D279N, D277, E224Q, D279, P306T, D277N, E224

## Figures

1 figure with captions in the complete paper: https://tomesphere.com/paper/PMC12183322/full.md

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Source: https://tomesphere.com/paper/PMC12183322