# Evaluation of Regeneration Potential of Bone Marrow–Derived Mesenchymal Stem Cells on Induced Damaged Submandibular Salivary Gland in Mice

## TL;DR

This study investigates whether bone marrow-derived stem cells can regenerate damaged salivary glands in mice, finding that timing affects their regenerative potential.

## Contribution

The study demonstrates the regenerative potential of BM-MSCs in irradiated salivary glands and highlights the importance of timing in treatment.

## Key findings

- BM-MSCs show regeneration potential in irradiated submandibular salivary glands.
- Timing of BM-MSC injection significantly affects regeneration outcomes.
- CD34 immunoexpression indicates improved microvessel density in treated groups.

## Abstract

Objectives
 The ultimate goal of stem cell (SC) transplantation is the regeneration of salivary gland function by transplanted SCs differentiating into salivary gland cells. Therefore, this study aimed to evaluate the regenerative capacity of bone marrow–derived mesenchymal stem cells (BM-MSCs) transplantation in irradiated mice using the immunohistochemical markers Ki-67 and CD34.

Material and Methods
 Four groups of male mice were included in the study. Group I (normal control) comprised six mice that were not subjected to gamma radiation. Group II comprised six irradiated mice that were not treated with BM-MSCs. Group III comprised 12 irradiated mice that were treated with intraglandular injection of labeled BM-MSCs into their submandibular salivary glands, 24 hours postradiation. Group IV comprised 12 irradiated mice that were treated with intraglandular injection of labeled BM-MSCs into their submandibular salivary glands, on day 11 postradiation.

Statistical Analysis
 Data were presented as mean and standard deviation. The different groups were compared using a one-way analysis of variance (ANOVA).

Results
 The ANOVA test revealed that the difference between all groups was extremely statistically significant (
p
 < 0.003), and Tukey's post hoc test revealed a statistically significant difference between group II and groups I, III, and IV included in the study regarding microvessel density of CD34 immunoexpression in different groups.

Conclusion
 BM-MSCs have a regeneration potential on induced damaged submandibular salivary glands in mice; time is an essential factor in the regeneration capacity of BM-MSCs.