# Unveiling the structure, function and dynamics of StmPr1 in Stenotrophomonas maltophilia virulence

## TL;DR

This paper investigates StmPr1, a key virulence factor in Stenotrophomonas maltophilia, revealing its structure and potential as a drug target.

## Contribution

The study provides novel structural and functional insights into StmPr1 and evaluates boron-based inhibitors like Bortezomib as potential therapeutics.

## Key findings

- StmPr1 exhibits autoproteolytic activity, producing a shortened active enzyme.
- Bortezomib shows potential as a therapeutic inhibitor against S. maltophilia.
- StmPr1's C-terminal extension is crucial for correct folding and stability.

## Abstract

The increase in infections caused by multi-resistant Gram-negative bacteria, like Stenotrophomonas maltophilia, has become a growing health crisis worldwide. S. maltophilia poses a risk because of its tendency to opportunistically infecting patience for example through colonization of catheters in hospital environments using its intrinsic resistance against multiple antibiotics. Through the COVID-19 pandemic it gained more prominence by being a key pathogen in respiratory co-infections. This study will present a structural analysis of StmPr1, S. maltophilia’s main virulence factor, an excreted serine protease. Our study outline structure and functional aspects of StmPr1, revealing a unique autoproteolytic activity resulting in a shortened version of the active enzyme. We also investigated the potential of two groups of peptide-based inhibitors, one being acetyl- and the other being boron-based inhibitors. The focus here lies on Bortezomib, a boron-based serine protease inhibitor, and its potential therapeutic use against S. maltophilia. We provide a structure-function analysis which includes X-ray crystallography data with resolutions ranging from 1.64 to 2.08 Å, molecular dynamic simulations and small-angle X-ray scattering (SAXS) experiments. These data provide a deeper understanding of StmPr1’s resilience and mechanisms, while highlighting the relevance of StmPr1’s C-terminal extension for correct folding and its stability. Moreover, it also shows that StmPr1 is promising target for further drug discovery investigations to identify compounds and drugs to treat S. maltophilia infections.

The online version contains supplementary material available at 10.1038/s41598-025-06177-5.