The Astrocyte Elevated Gene- 1 Expression and other Risk Factors for Different Varieties of Diabetic Optic Neuropathy in Egyptian Patients with Diabetic Retinopathy

TL;DR

This study investigates how AEG-1 gene expression and other factors contribute to diabetic optic neuropathy in Egyptian patients with diabetic retinopathy.

Contribution

The study identifies AEG-1 gene expression and metabolic factors as novel risk factors for different types of diabetic optic neuropathy.

Findings

AEG-1 mRNA and protein levels are significantly higher in diabetic patients with optic neuropathy.

AEG-1 mRNA and protein are significant risk factors for all types of diabetic optic neuropathy.

Blood glucose and diabetes duration are linked to specific optic neuropathy subtypes.

Abstract

To assess the role of the mRNA and protein expression of Astrocyte Elevated Gene- 1 (AEG-1) and other possible risk factors for diabetic optic neuropathy (DON) in Egyptian patients with diabetic retinopathy (DR). This case–control study constituted 450 diabetic patients divided into three groups: Group 1 included 150 DR patients with any sign of DON (diabetic papillopathy (DP), non-arteritic anterior ischemic optic neuropathy (AION), and optic atrophy (OA)). Group 2 included 150 DR patients without any sign of DON. Group 3 included 150 sex and age-matched diabetic patients without any sign of DR or DON. The expression level of AEG-1 mRNA was assessed by real-time PCR. Serum AEG-1 was determined by ELISA. Multivariate logistic regression analysis was conducted to assess the DON risk factors. The mRNA expression of the AEG-1 gene and AEG-1 protein levels were significantly increased (P < 0.001) in diabetic patients with (group 1) than without (group 2) DON. ROC curve analysis showed that the mRNA level of the AEG-1 gene exhibited a 70% specificity and a 79.33% diagnostic sensitivity. The diagnostic sensitivity of the serum concentration of AEG-1 was 84.67%, while the specificity was 70%. Multivariate regression analysis showed that the risk factors for DON were 2hpp blood sugar, HbA1c, serum cholesterol, AEG-1 gene's mRNA expression, and AEG-1 protein. Serum cholesterol, the AEG-1 gene's mRNA expression, and the AEG-1 protein are risk factors for all varieties of DON. While the blood glucose levels favour the development of DP, control of diabetes favours the occurrence of AION. Lastly, diabetes duration is more related to the presence of OA.

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