Acquired CRISPR spacers and rhamnose-glucose polysaccharide defects confer resistance to Streptococcus mutans phage ɸAPCM01

TL;DR

This study explores how Streptococcus mutans develops resistance to a specific phage, finding that CRISPR spacers and mutations in polysaccharide genes play key roles.

Contribution

The study identifies new CRISPR spacers and rhamnose-glucose polysaccharide gene mutations that confer phage resistance in S. mutans.

Findings

Ten resistant mutants acquired CRISPR spacers targeting the phage, with 18 new spacers identified.

Mutations in rhamnose-glucose polysaccharide biosynthetic genes caused phage adsorption defects.

Rgp mutations led to severe cell division and biofilm formation issues, reducing pathogenic potential.

Abstract

Streptococcus mutans is commonly associated with the development of dental caries worldwide. Due to their specificity for S. mutans, phage represents a promising avenue for future targeted therapeutic strategies. In this study, we investigated how phage resistance develops in S. mutans. As a model phage, we used ɸAPCM01, which is known to infect a serotype e strain. We isolated and sequenced the genomes of 15 spontaneous resistant mutants and found that 10 had acquired novel clustered regularly interspaced short palindromic repeats (CRIPSR) spacers targeting the phage, with a total of 18 new spacers identified. Additionally, eight strains contained mutations in rhamnose-glucose polysaccharide biosynthetic genes, three of which also acquired spacers. Only the rgp mutants exhibited defects in phage adsorption, supporting the role of these cell surface glycans as the phage receptor. Mutations in rgpF and the newly identified gene rgpX led to severe cell division defects and impaired biofilm formation, the latter of which was also shared by an rgpD mutant. Thus, rgp mutations confer phage resistance but impose severe fitness costs, limiting pathogenic potential. Surprisingly, we found that ɸAPCM01 was capable of binding to and injecting its genome into UA159, a model serotype c strain. However, UA159 was resistant to infection due to an unknown post-entry defence mechanism. Consequently, ɸAPCM01 has the potential to infect both major serotypes associated with dental caries.

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