Study on resting-state functional connectivity characteristics under hypnosis using functional near-infrared spectroscopy

TL;DR

This study used fNIRS to explore how hypnosis affects brain connectivity in the prefrontal cortex, finding specific changes in functional connections during hypnotic states.

Contribution

The study provides new empirical evidence on how hypnosis modulates resting-state functional connectivity in the prefrontal cortex using fNIRS.

Findings

Hypnosis increased functional connectivity in six prefrontal region pairs with statistical significance.

Hypnosis decreased functional connectivity in four prefrontal region pairs with statistical significance.

fNIRS is shown to be a viable method for studying brain activity changes during hypnosis.

Abstract

Numerous studies suggest that hypnosis has significant potential in mental health and cognitive disorder treatments. However, the mechanisms by which hypnosis influences brain activity and functional network connectivity remain unclear. This study employed functional near-infrared spectroscopy (fNIRS) to investigate resting-state functional connectivity (rsFC) under hypnosis.

Twenty-six healthy college students participated in the study. Resting-state oxygenated hemoglobin (HbO) data were collected from the prefrontal cortex (PFC) during both control aware and hypnotic states. Functional connectivity strengths between these states were analyzed to assess changes in brain activity associated with deep hypnosis.

A total of 55 paired samples t-tests were conducted across 11 regions of interest (ROIs), revealing statistically significant differences (p < 0.05) in functional connectivity strength between the control state and hypnotic state in 10 paired comparisons. Increased connectivity during hypnosis (6 pairs): LBA9-RBA10 (t = −2.672, p = 0.013), LBA6-RBA46 (t = −2.948, p = 0.007), LBA46-RBA46 (t = −2.516, p = 0.019), RBA8-RBA46 (t = −2.689, p = 0.013), RBA9-RBA46 (t = −2.090, p = 0.047), LBA10-RBA10 (t = −2.315, p = 0.029); Decreased connectivity during hypnosis (4 pairs): LBA9-LBA45 (t = 2.064, p = 0.049), LBA6-LBA45 (t = 3.151, p = 0.004), LBA8-LBA45 (t = 2.438, p = 0.022), LBA8-RBA9 (t = 2.085, p = 0.047). No significant differences were observed in connectivity strength between other ROI pairs.

Hypnosis appears to modulate the function of the DLPFC, PFC, and related regions, enhancing specific brain network functional connectivity. This preliminary study demonstrates that resting-state functional connectivity analysis using fNIRS is a valuable approach for studying brain activity during hypnosis.

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