Correction: A highly efficient heterologous expression platform to facilitate the production of microbial natural products in Streptomyces
Xiuling Wang, Ping Lin, Qiyao Shen, Xueyan Feng, Shouying Xu, Qijun Zhang, Yang Liu, Cailing Ren, Daojing Yong, Qiong Duan, Liujie Huo, Youming Zhang, Gang Li, Jun Fu, Ruijuan Li
Abstract
Genes, proteins, chemicals, diseases, species, mutations and cell lines named across the full text — each resolved to its canonical identifier and authoritative record.
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Taxonomy
TopicsMicrobial Natural Products and Biosynthesis · Chemical Synthesis and Analysis · Click Chemistry and Applications
Microbial Cell Factories (2025) 24:105
10.1186/s12934-025-02722-z
In this article the wrong figure appeared as Fig. 2; the figure should have appeared as shown below.
Incorrect Fig. 2.
Fig. 2. Characterization of the engineered E. coli strains. (A) Conjugation frequency of plasmid pBAC-sal-phiC31-apra-oriT in transfer from eleven engineered E. coli strains to S. coelicolor A3(2). Three of the engineered strains were derivatives of E. coli GB2005 (purple), with four engineered strains derived each from E. coli DH5G (orange) and E. coli GB2006 (blue). N represents no data. (B) Electroporation efficiency of four engineered strains and E. coli ET12567 as determined by the number of colonies grown after transfer of plasmid pBAC-sal-phiC31-apra-oriT (116 kb). (C) Recombination efficiency of E. coli GB05-recETtra-αβγ, DH5G-Gtra-αβγ, and GB06-DLP12tra-αβγ. The correct recombinant was not obtained in GB06-DLP12tra-αβγ. (D) Internal recombination ratio of pBAC-cm-ampF-kan-ampR-repeat in four engineered E. coli strains and ET12567. Error bars, SD; n = 3; ns, p > 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001
Correct Fig. 2.
Fig. 2. Characterization of the engineered E. coli strains. (A) Conjugation frequency of plasmid pBAC-sal-phiC31-apra-oriT in transfer from eleven engineered E. coli strains to S. coelicolor A3(2). Three of the engineered strains were derivatives of E. coli GB2005 (purple), with four engineered strains derived each from E. coli DH5G (orange) and E. coli GB2006 (blue). N represents no data. (B) Electroporation efficiency of four engineered strains and E. coli ET12567 as determined by the number of colonies grown after transfer of plasmid pBAC-sal-phiC31-apra-oriT (116 kb). (C) Recombination efficiency of E. coli GB05-recETtra-αβγ, DH5G-Gtra-αβγ, and GB06-DLP12tra-αβγ. The correct recombinant was not obtained in GB06-DLP12tra-αβγ. (D) Internal recombination ratio of pBAC-cm-ampF-kan-ampR-repeat in four engineered E. coli strains and ET12567. Error bars, SD; n = 3; ns, p > 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001
- The statement in the Result section-“however, recombinants were not obtained with GB06-DLP12tra-αβγ (Fig. 2C)”-should be revised to: “While the recombination efficiency of GB06-DLP12tra-αβγ was inferior to that of DH5G-Gtra-αβγ or GB05-recETtra-αβγ, correct recombinants were indeed generated (Fig. 2C).”