# Protocol for the isolation and characterization of mouse alveolar bone marrow hematopoietic stem cells

## TL;DR

This paper provides a detailed protocol for isolating and identifying hematopoietic stem cells from the alveolar bone marrow of mice.

## Contribution

A new method for isolating HSCs from a specific alveolar bone marrow niche using dissection and flow cytometry.

## Key findings

- The protocol combines anatomical dissection with flow cytometry to isolate HSCs.
- The alveolar bone marrow niche contains an enriched population of HSCs.
- The method allows for precise characterization of HSCs from this specific niche.

## Abstract

Here, we present a protocol for isolating and characterizing hematopoietic stem cells (HSCs) from mouse alveolar bone marrow. We describe steps for combining anatomical dissection of the mandibular alveolar region with flow cytometry analysis for identifying HSCs. The protocol enables the isolation of HSCs from a specific bone marrow niche harboring an enriched HSC population.

For complete details on the use and execution of this protocol, please refer to Niizuma et al.1

•Protocol for isolating hematopoietic stem cells from mouse alveolar bone marrow•Instructions for anatomical dissection of the mandibular alveolar region•Procedure for identifying HSCs from this enriched niche using flow cytometry

Protocol for isolating hematopoietic stem cells from mouse alveolar bone marrow

Instructions for anatomical dissection of the mandibular alveolar region

Procedure for identifying HSCs from this enriched niche using flow cytometry

Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.

Here, we present a protocol for isolating and characterizing hematopoietic stem cells (HSCs) from mouse alveolar bone marrow. We describe steps for combining anatomical dissection of the mandibular alveolar region with flow cytometry analysis for identifying HSCs. The protocol enables the isolation of HSCs from a specific bone marrow niche harboring an enriched HSC population.