Evaluation of Acute and Subchronic Oral Toxicity of Copra Meal Hydrolysate: A Novel Candidate for Prebiotic in Sprague Dawley Rats
Jiraporn Tangthong, Francis Ayimbila, Massalin Nakphaichit, Suttipun Keawsompong
TL;DR
This study shows that copra meal hydrolysate is safe for use as a prebiotic in rats, with no harmful effects observed at tested doses.
Contribution
The study provides the first systematic safety evaluation of copra meal hydrolysate as a prebiotic in rats.
Findings
The LD50 of CMH was over 2000 mg/kg, indicating low acute toxicity.
No adverse effects were observed in subchronic toxicity tests at doses up to 1.0 mg/kg.
CMH had a NOAEL of 1.0 mg/kg for both male and female rats.
Abstract
Copra meal hydrolysate (CMH) with high protein and mannooligosaccharides (MOS) was derived by β-mannanase hydrolysis. CMH has been shown to elicit health benefits via prebiotic properties. However, a systematic examination of its safety is required before effective utilization. This study assessed CMH oral acute toxicity at a single dose of 2000 mg/kg for 14 consecutive days, while a subacute toxicity test was conducted by daily oral administration of CMH at doses of 0.25, 0.5 and 1.0 mg/kg for 90 days using Sprague Dawley rats and following OECD guidelines 423 and 408. The acute toxicity study showed that the LD50 of CMH was over 2000 mg/kg since no mortality or abnormal clinical signs were observed at this dose. The subacute toxicity results showed that CMH did not induce any abnormalities in body weight, food and water consumption, clinical signs, haematology, clinical chemistry,…
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Taxonomy
TopicsProbiotics and Fermented Foods · Protein Hydrolysis and Bioactive Peptides · Proteins in Food Systems