Efficacy of NAMPT inhibition in T-cell acute lymphoblastic leukemia

TL;DR

This study explores the potential of NAMPT inhibition as a new treatment for T-cell acute lymphoblastic leukemia, showing promising results in both human and mouse models.

Contribution

The study identifies NAMPT inhibition as a novel and effective therapeutic strategy for T-cell acute lymphoblastic leukemia.

Findings

The NAMPT inhibitor FK866 showed the highest cytotoxicity in human and mouse T-ALL cell lines and patient samples.

In PDX mouse models, FK866 significantly reduced disease burden and prolonged survival of leukemic mice.

The findings support NAMPT inhibition as a promising treatment strategy for T-ALL.

Abstract

Novel agents targeting upregulated signaling pathways are needed to improve outcomes in T-cell acute lymphoblastic leukemia (T-ALL), since conventional cytotoxic chemotherapy regimens have reached the limits of tolerability. We identified upregulated, targetable signaling pathways common to both human T-ALL samples and a KrasLSL-G12D/+.Mb1Cre/+ murine model of T-ALL. We found the NAMPT inhibitor FK866 had the greatest cytotoxicity of a panel of small molecule inhibitors tested in human and mouse T-ALL cell lines, and in patient derived xenograft (PDX)-expanded T-ALL patient samples. We subsequently tested FK866 in vivo in PDX mouse models of T-ALL, and found that it significantly reduced the peripheral blood disease burden and prolonged the survival of leukemic mice (median survival of 60.5 vs 21 days, p = 0.0007). This screen for targetable pathways in T-ALL generated in vitro and in vivo preclinical data supporting NAMPT inhibition as a promising strategy for the treatment of T-ALL.

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