Methyltransferase gene-mediated antiproliferative effect of 1,25(OH)2D3 on neuroblastoma cells

TL;DR

This study shows that calcitriol reduces the growth and migration of neuroblastoma cells by affecting methyltransferase genes.

Contribution

The study demonstrates that calcitriol decreases methyltransferase gene expression in neuroblastoma cells, suggesting a novel anticancer mechanism.

Findings

1,25-Dihydroxyvitamin D3 reduced cell viability with an IC50 of 265.6 nM.

1,25-Dihydroxyvitamin D3 decreased DNA methyltransferase 3 alpha and beta gene expression.

1,25-Dihydroxyvitamin D3 inhibited cell migration in neuroblastoma cells.

Abstract

1,25-Dihydroxyvitamin D3 is the body's active form of vitamin D and is also known as calcitriol. It has an effect on the body's immune system and is involved in the regulation of cell growth, differentiation, and apoptosis.

The effect of 1,25-Dihydroxyvitamin D3 on the viability of human neuroblastoma human neuroblastoma cell line cells was investigated by 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide assay, the effect on migration was investigated by wound healing assay, and methyltransferase genes (DNA methyltransferase 3 alpha and DNA methyltransferase 3 beta) and phosphatase and tensin homolog gene expression levels were investigated by real time polymerase chain reaction method.

As a result of the 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide assay, 1,25-Dihydroxyvitamin D3 IC50:265.6 nM was found. It was observed that 100 and 265.6 nM applied to human neuroblastoma cell line cells had an antiproliferative effect. In addition, DNA methyltransferase 3 alpha and DNA methyltransferase 3 beta genes, which are effective as methyltransferases, caused a change in expression levels (p<0.05), while phosphatase and tensin homolog expression levels did not change (p>0.05).

1,25-Dihydroxyvitamin D3 showed antiproliferative, antimigratory activity on neuroblastoma human neuroblastoma cell line cells and decreased the expression levels of genes involved in methylation, which may be evidence of its anticancer effect.

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