# Development and validity of type II sulcus vocalis in excised canine larynx

## TL;DR

This study created a model of type II sulcus vocalis in canine larynges and validated it using acoustic, aerodynamic, and histological methods.

## Contribution

The study introduces a new experimental model for type II sulcus vocalis to study its pathology and treatment options.

## Key findings

- The model showed significant changes in phonation threshold pressure, fundamental frequency, jitter, shimmer, and harmonics-to-noise ratio.
- The model's histology and laryngeal videostroboscopy results resembled those of patients with sulcus vocalis.
- The model can be used to evaluate and compare treatments for sulcus vocalis.

## Abstract

This study aims to develop a sulcus vocalis model in the excised canine larynx and to investigate the validity of the model.

Type II sulcus vocalis was created by continuous intradermal suture in six excised canine larynges. We investigated the validity of the model based on phonatory (aerodynamic and acoustic) measures, laryngeal videostroboscopy (LVS), and histological examination. The aeroacoustic parameters included phonation threshold pressure (PTP), fundamental frequency (F
o), jitter %, shimmer %, and harmonics‐to‐noise ratio (HNR).

In all the experimental specimens, there were significant increases in the PTP ([6.61 ± 1.66] cm H2O, p < 0.01), the F
o ([106.48 ± 49.59] Hz, p = 0.003), the jitter ([0.76 ± 0.42]%, p = 0.007), the shimmer ([6.87 ± 2.99%, p = 0.002]), while the HNR decreased ([5.71 ± 4.68] dB, p = 0.031), compared to that of the untreated canine larynges. The estabilished model simulated the histology with type Ⅱ sulcus.

Type Ⅱ sulcus vocalis was successfully created in excised canine larynx. The aeroacoustic and LVS analysis of this model resembled the characteristics of patients with sulcus vocalis. The model could be helpful to elucidate the pathology of the phonation, and evaluate and compare the treatments for sulcus vocalis.

Significant findings of the study: This study established a type II sulcus vocalis model in excised canine larynx.What this study adds: This study provided an experimental model to elucidate the pathology of the phonation, evaluate and compare the treatments for sulcus vocalis.

Significant findings of the study: This study established a type II sulcus vocalis model in excised canine larynx.

What this study adds: This study provided an experimental model to elucidate the pathology of the phonation, evaluate and compare the treatments for sulcus vocalis.