# Whole-genome sequencing of the ant Crematogaster osakensis (Hymenoptera: Formicidae: Myrmicinae)

## TL;DR

This paper presents the whole-genome sequence of the ant Crematogaster osakensis to study queen-specific traits like reproduction and longevity.

## Contribution

The study provides a high-quality genome and gene annotations for C. osakensis to support future molecular research on ant reproduction.

## Key findings

- The genome size of C. osakensis is 284 Mb with 0.26% heterozygosity.
- 16,053 protein-coding genes were identified through genome annotation.
- Reanalysis of transcriptome data revealed highly expressed genes in reproductive organs of queens and males.

## Abstract

The ant Crematogaster osakensis (Hymenoptera: Formicidae: Myrmicinae) serves as a valuable model organism for investigating queen-specific traits, such as reproductive capacity and longevity, at the cellular and molecular levels. To support future research on queen traits using molecular techniques, including genome editing and multi-omics data analyses, we performed whole-genome sequencing of this species. The genome size of C. osakensis was estimated to be 284 Mb, with a heterozygosity of 0.26%. Annotation revealed the presence of 16,053 protein-coding genes. Furthermore, using the coding sequences annotated in this study, we reanalyzed previously obtained transcriptome data to identify highly expressed genes in the reproductive organs of queens and males. This data will contribute to a deeper understanding of the genetic mechanisms underlying reproductive strategies in ants.