# Genome Sequence of the Cluster EF Bacteriophage TinyMiny, Isolated using Microbacterium foliorum

**Authors:** Iain Duffy, Juan Jimenez, Luis Gomez, Alexa Keeler, Aisha Ambrose, Moises Guadarrama, Hailey Kerns, Rachael Martin, Jade Moorehead, Arrianne Orgill, Brianna Reiber, Aisha Severe, Leslie Sivley, Genesis Valentin, John Duncan

PMC · DOI: 10.17912/micropub.biology.001488 · microPublication Biology · 2025-05-29

## TL;DR

This paper describes the genome of a new bacteriophage called TinyMiny, isolated from soil using a specific bacterium.

## Contribution

The paper presents the genome sequence and classification of the newly isolated bacteriophage TinyMiny into the EF cluster.

## Key findings

- TinyMiny has a 57,042 base pair double-stranded DNA genome.
- The phage encodes 86 genes and is classified into the EF cluster based on gene content similarity.
- It was isolated using Microbacterium foliorum NRRL B-24224 from a soil sample in Maryland.

## Abstract

The bacteriophage TinyMiny was isolated from a soil sample collected on the campus of the University of Maryland Baltimore County, in Baltimore, Maryland, using the bacterium
Microbacterium foliorum
NRRL B-24224. TinyMiny is presumed to be a lytic bacteriophage, consisting of a double-stranded DNA genome 57,042 base pairs in length and encoding 86 genes. Based on gene content similarity to actinobacteriophages, TinyMiny is assigned to the EF cluster.

## Linked entities

- **Species:** Microbacterium foliorum (taxon 104336)

## Full text

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## Figures

1 figure with captions in the complete paper: https://tomesphere.com/paper/PMC12163623/full.md

## References

18 references — full list in the complete paper: https://tomesphere.com/paper/PMC12163623/full.md

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Source: https://tomesphere.com/paper/PMC12163623