# Development and characterization of a saturated transposon mutant library of Salmonella enterica serovar Enteritidis LA5

**Authors:** Jérôme Trotereau, Anne-Sophie Huguet, Romain Jouan, Florent Kempf, Delphine Naquin, Catherine Schouler, Philippe Velge, Peter Mergaert, Isabelle Virlogeux-Payant

PMC · DOI: 10.1128/mra.01319-24 · Microbiology Resource Announcements · 2025-05-27

## TL;DR

This paper describes creating a comprehensive genetic library in Salmonella to study essential genes and virulence factors.

## Contribution

A saturated transposon mutant library was developed for Salmonella Enteritidis LA5 to identify essential and virulence-related genes.

## Key findings

- A saturated transposon mutant library was successfully created in Salmonella Enteritidis LA5.
- The study identified essential genes required for growth in rich medium.
- The library serves as a functional genomics tool for studying virulence determinants.

## Abstract

We created and characterized a saturated Tn-seq mutant library in Salmonella enterica subsp. enterica ser. Enteritidis strain LA5. The set of essential genes after culture in rich medium was determined. This functional genomics tool will aid in identifying the roles of chromosomal and plasmid virulence determinants.

## Full-text entities

- **Chemicals:** Tn (MESH:C009497), enterica (-)
- **Species:** Salmonella enterica (species) [taxon 28901]

## Full text

_Full body text omitted from this summary view._ Fetch the complete paper as Markdown: https://tomesphere.com/paper/PMC12160557/full.md

## Figures

1 figure with captions in the complete paper: https://tomesphere.com/paper/PMC12160557/full.md

## References

17 references — full list in the complete paper: https://tomesphere.com/paper/PMC12160557/full.md

---
Source: https://tomesphere.com/paper/PMC12160557