# Draft genome sequence of multidrug-resistant Bacillus subtilis strain Hakim RU_LJ isolated from lacchi juice in Bangladesh

**Authors:** Muhib Ullah Khan, Md. Arif-Uz-Zaman Polash, Nusrat Zahan, Md. Mottaleb Ali, Nilima Rubaba Azad, Subir Sarker, Md. Hakimul Haque

PMC · DOI: 10.1128/mra.00278-25 · Microbiology Resource Announcements · 2025-05-27

## TL;DR

The paper presents the draft genome of a drug-resistant Bacillus subtilis strain from Bangladesh, revealing resistance and virulence genes.

## Contribution

The study provides a new multidrug-resistant genome from lacchi juice with detailed resistance and virulence gene profiles.

## Key findings

- The genome has 17 antibiotic resistance genes and 10 virulence factor genes.
- Six CRISPR arrays and 19 prophages were identified in the genome.
- The assembled genome has a GC content of 43.5% and 50.33× coverage.

## Abstract

This report details the draft genome sequence of the multidrug-resistant Bacillus subtilis strain Hakim RU_LJ derived from lacchi juice. The assembled genome spans 4.03 Mb, with 50.33× coverage and a GC content of 43.5%. Analysis revealed six CRISPR arrays, 19 prophages, 17 antibiotic resistance genes, and 10 virulence factor genes.

## Linked entities

- **Species:** Bacillus subtilis (taxon 1423)

## Full-text entities

- **Species:** Bacillus subtilis (species) [taxon 1423]

## Full text

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## Figures

1 figure with captions in the complete paper: https://tomesphere.com/paper/PMC12160527/full.md

## References

22 references — full list in the complete paper: https://tomesphere.com/paper/PMC12160527/full.md

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Source: https://tomesphere.com/paper/PMC12160527