# Draft genome sequences of a novel Escherichia coli K-12 MG1655 L-form strain and its evolved variant

**Authors:** Marjolein E. Crooijmans, Johannes H. de Winde, Dennis Claessen

PMC · DOI: 10.1128/mra.01283-24 · Microbiology Resource Announcements · 2025-05-27

## TL;DR

This study presents draft genomes of an Escherichia coli strain that can grow without a cell wall and a variant that grows better.

## Contribution

The paper introduces novel draft genome sequences of an E. coli L-form strain and its evolved variant with improved growth.

## Key findings

- The L-form E. coli strain can proliferate without a cell wall.
- An evolved variant of the L-form strain shows enhanced growth capabilities.
- Draft genome sequences reveal genetic adaptations for cell wall-independent growth.

## Abstract

L-forms are bacterial variants capable of proliferating without a cell wall. In this evolution study, we present draft genome sequences of a novel Escherichia coli K-12 L-form strain and an evolved variant that exhibits enhanced growth. These draft sequences provide insights into the genetic adaptations associated with cell wall-independent growth.

## Linked entities

- **Species:** Escherichia coli K-12 (taxon 83333)

## Full-text entities

- **Species:** Escherichia coli str. K-12 substr. MG1655 (no rank) [taxon 511145]

## Full text

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## Figures

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## References

10 references — full list in the complete paper: https://tomesphere.com/paper/PMC12160447/full.md

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Source: https://tomesphere.com/paper/PMC12160447