# Effect of Graphene Oxide Quantities on Microhardness of Cured-Surface Coating Agents

## TL;DR

This study shows that adding small amounts of graphene oxide to dental coatings can significantly increase their surface hardness.

## Contribution

The study identifies optimal graphene oxide concentrations that enhance microhardness in different dental coating agents.

## Key findings

- Adding 0.3–0.7 wt% GO increased surface hardness in ReG, CoI, and AdA compared to controls.
- Optimal GO concentrations varied by material, with OpA showing increased hardness at 0.05–0.3 wt% GO.
- Higher GO concentrations (1–10 wt%) led to decreased microhardness in most tested materials.

## Abstract

This study aimed to investigate the impact of varying concentrations of graphene oxide (GO) combined with two surface coating agents (SCAs) and two dental adhesives (DAHs) used as SCAs on microhardness. Two SCAs, Resin Glaze (ReG) and Coat-It (CoI) (Shofu Inc., Kyoto, Japan), along with two DAHs, AdperTM ScotchbondTM Multi-purpose Adhesive (AdA) (3M ESPE, Seefeld, Germany) and OptiBondTM FL Adhesive (OpA) (Kuraray Noritake Dental Inc., Okayama, Japan), were tested. The ten concentrations of GO—0 wt % (control), 0.05 wt %, 0.1 wt %, 0.3 wt %, 0.5 wt %, 0.7 wt %, 1 wt %, 2 wt %, 5 wt %, and 10 wt %—were incorporated into the SCAs and DAHs to create the experimental formulations. These mixtures underwent centrifugation for homogenization, followed by sonication for dispersion. The mixture was poured into the 3D-printed resin mold (10 mm in diameter and 1 mm in height) and then cured with a light curing unit for 180 s. The cured specimens were then kept in distilled water at 37 ± 1 °C for 24 h. All specimens were then subjected to evaluation of their microhardness properties using a Knoop hardness testing machine. Data were collected, and the statistical analysis was conducted using Two-way ANOVA followed by Tukey’s post-hoc tests at a 0.05 level of significance. According to the results, surface hardness was significantly increased (p < 0.05) when 0.3–0.7 wt % of GO was added to ReG, CoI, and AdA, compared to the control group. However, surface hardness was significantly increased (p < 0.05) when 0.05–0.3 wt % of GO was added to OpA compared to the control group. In the control groups, the microhardness of OpA was significantly higher than that of the other groups (p < 0.05). In the 0.1 wt % groups, the microhardness of OpA was significantly higher than that of the other groups (p < 0.05). At 0.5 wt %, ReG, CoI, and AdA showed significantly higher microhardness compared to their respective control groups (p < 0.05). In the 1–10 wt % groups, the microhardness of ReG, CoI, and AdA demonstrated a gradual, significant decrease compared to the 0.7 wt % groups. Whereas in the 0.5–10 wt % groups, the microhardness of OpA showed a significant gradual decrease compared to the 0.3 wt % group. In summary, the optimal GO concentration could improve the surface hardness of ReG, CoI, AdA, and OpA.