Integrated Metabolomics and Network Pharmacology to Reveal the Mechanisms of Forsythia suspensa Extract Against Respiratory Syncytial Virus
Haitao Du, Jie Ding, Yaxuan Du, Xinyi Zhou, Lin Wang, Xiaoyan Ding, Wen Cai, Cheng Wang, Mengru Zhang, Yi Wang, Ping Wang
TL;DR
This study explores how Forsythia suspensa extract fights respiratory syncytial virus by combining metabolomics and network pharmacology to identify key compounds and pathways.
Contribution
The study introduces an integrated approach combining metabolomics and network pharmacology to uncover the antiviral mechanisms of Forsythia suspensa extract.
Findings
Twenty-five components in Forsythia suspensa were identified, with 43 effective components and 113 potential targets for anti-RSV activity.
Rutin, quercetin, and kaempferol were confirmed to modulate Toll-like receptor and arachidonic acid metabolism pathways.
FS reduced lung inflammation in RSV-infected mice by downregulating relevant protein expression.
Abstract
To investigate the therapeutic impact of Forsythia suspensa extract (FS) on RSV-infected mice and explore its antiviral pharmacodynamic foundations. Methods: An integrated analytical approach, combining UPLC-Q-TOF/MS with network pharmacology, was employed to analyze and identify the chemical constituents in FS, particularly those exhibiting antiviral properties against RSV. The study integrated network pharmacology and metabolomics for further analysis, and molecular docking and interaction experiments were conducted to validate the pharmacodynamic mechanisms. Finally, an RSV pneumonia mouse model was employed to evaluate the therapeutic influence of FS, including pathological and immunohistochemistry assessments. Twenty-five components in FS were identified through UPLC-Q-TOF/MS analysis. Integrated network pharmacology data revealed 43 effective components and predicted 113 potential…
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Taxonomy
TopicsRespiratory viral infections research · Phytochemistry and Biological Activities · Gut microbiota and health