Photodynamic Evaluation of Synthesized Chlorin-Desthiobiotin Conjugate with Chemotherapeutic Drugs in Triple-Negative Breast Cancer Cells In Vitro and in Hydra Organisms In Vivo
Bailey N. Rutkowski, Meden F. Isaac-Lam
TL;DR
This study explores a new chlorin-based compound for photodynamic therapy in triple-negative breast cancer, showing promising results when combined with certain chemotherapy drugs.
Contribution
The novel use of a chlorin-desthiobiotin conjugate in combination with chemotherapeutics for photodynamic therapy in TNBC and its evaluation in hydra as an in vivo model.
Findings
CDBTN combined with paclitaxel or fluorouracil showed photodynamic activity against TNBC cells.
CDBTN-fluorouracil combination demonstrated synergy in vitro.
In hydra, CDBTN-cisplatin combination was more phototoxic than other combinations.
Abstract
In this article, the synthesis and characterization of chlorin-based photosensitizers for potential applications in photodynamic therapy (PDT) of triple-negative breast cancer (TNBC) are described. The photodynamic efficacy of the synthesized chlorin-desthiobiotin (CDBTN) conjugate and its zinc and indium complexes were compared with the starting unconjugated precursor methyl pheophorbide, and assessed in a TNBC cell line in vitro. The chlorin-desthiobiotin complex aims to target the vitamin receptors upregulated in malignant cancer cells. The synthesized CDBTN was combined with chemotherapeutic agents (paclitaxel, cisplatin or fluorouracil) to evaluate their binary photodynamic efficacy. Cell survival assay in vitro indicated that the chlorin-vitamin conjugate CDBTN—alone and in combination with paclitaxel or fluorouracil—is photoactive against the TNBC cell line, but not when combined…
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Taxonomy
TopicsPhotodynamic Therapy Research Studies · Nanoplatforms for cancer theranostics · Hemoglobin structure and function