# Therapeutic Potential of Human Amniotic Membrane-Derived Mesenchymal Stem Cell Conditioned Medium in Combating Oxidative Stress and Age-Related Female Infertility

## TL;DR

This study explores how a substance derived from human amniotic membrane stem cells can reduce oxidative stress and improve fertility in aged female mice.

## Contribution

The novel contribution is demonstrating the antioxidative and anti-aging effects of AMSC-CM in aged female reproductive systems.

## Key findings

- AMSC-CM reduced oxidative stress in reproductive organs of aged mice at RNA and protein levels.
- AMSC-CM treatment improved age-related changes in reproductive hormones.
- AMSC-CM promoted gene expression related to anti-aging and female reproduction in aged mice.

## Abstract

Oxidative stress is a crucial factor accelerating the age-related functional deterioration of reproductive organs and fertility. Recently, human amniotic-membrane-derived mesenchymal stem cells (AMSCs) have emerged as a promising source with notable potential to reduce oxidative damage and support tissue regeneration. This study investigates the impact of the intravenous administration of human amniotic membrane-derived mesenchymal stem cell conditioned medium (AMSC-CM) on oxidative stress and reproductive competence in aged female mice. Antioxidative effects of human AMSC-CM were found in the reproductive organs of aged mice at both the RNA and protein levels. Human AMSC-CM positively regulated age-dependent changes in reproductive hormones, comparable to those observed in younger mice. RNA sequencing analysis revealed alterations in ovarian and uterine gene expression in aged mice showing that AMSC-CM treatment promotes the expression of genes essential for anti-aging, energy metabolism, and female reproductive processes. These findings highlight the potential of human AMSC-CM as a therapeutic strategy with anti-aging and antioxidative effects against age-related female infertility.