The transcriptional regulator VAL1 promotes Arabidopsis flowering by repressing the organ boundary genes BOP1 and BOP2

TL;DR

The VAL1 protein helps Arabidopsis plants flower by suppressing genes that control organ boundaries, and this happens under both long and short days.

Contribution

VAL1 is shown to promote flowering by epigenetically repressing BOP1 and BOP2, expanding its known regulatory role.

Findings

VAL1, not VAL2, is essential for flowering under both long and short days.

VAL1 represses BOP1 and BOP2 genes epigenetically to induce flowering.

Exogenous gibberellin bypasses the delayed flowering in val1 mutants.

Abstract

The transition to reproductive development is a critical step in the plant lifecycle and relies on the integration of intrinsic and environmental signals. Several different pathways controlling flowering time function downstream of the perception of environmental cues such as day length (photoperiodic pathway) and seasonal temperature (vernalization and ambient temperature pathways). In addition, the phytohormone gibberellin (GA) induces the floral transition under noninductive photoperiod. In the model plant Arabidopsis (Arabidopsis thaliana), the transcriptional repressor VIVIPAROUS1/ABSCISIC ACID INSENTIVE3 (ABI3)-LIKE1 (VAL1) triggers the stable repression of the floral repressor FLOWERING LOCUS C (FLC) during vernalization. However, the involvement of VAL1 in other flowering pathways remains unclear. In this work, we combined genetic and transcriptomic approaches to investigate the requirement of VAL1 for flowering activation under different day lengths. We found that VAL1, but not its sister protein VAL2, is required to induce the floral transition both under long and short days. The delayed flowering time of val1 mutant plants was fully bypassed by exogenous GA application. We demonstrated that VAL1-mediated induction of flowering occurs partially via the direct epigenetic repression of the organ boundary genes BLADE-ON-PETIOLE1 (BOP1) and BOP2. Our work thus expands the repertoire of VAL target genes and further demonstrates the pleiotropic role of VAL factors in regulating Arabidopsis development.

The transcriptional repressor VAL1 induces Arabidopsis flowering time by restricting the expression of the organ boundary genes BOP1 and BOP2 both under long- and short-day photoperiods.

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