# Production of an extract rich in alpha-tomatine from green tomatoes by subcritical water

## TL;DR

This paper shows how subcritical water extraction can efficiently produce a tomatine-rich extract from green tomatoes, which has potential uses in cosmetics and pharmaceuticals.

## Contribution

The study introduces an efficient and sustainable method for extracting alpha-tomatine from green tomatoes using subcritical water.

## Key findings

- SWE extracted about 200 mg tomatine/100 g tomatoes, higher than conventional methods.
- Extracts showed significant antioxidant activity and high saponin content.
- Fresh tomatoes had higher carbohydrate content than frozen ones.

## Abstract

Green tomatoes, a by-product of agro-food industry, are rich in the glycoalkaloid alpha-tomatine. This compound presents health benefits including anti-inflammatory and fungicide properties. Subcritical water extraction (SWE), a green and sustainable process, was used to obtain a tomatine-rich extract from fresh or frozen tomatoes. SWE extracted ca 200 mg tomatine/100 g tomato, an amount higher than conventional methods, at a temperature of 190°C and a residence time of 15 min. Green tomatoes' SWE extracts were characterized in terms of their composition and antioxidant activity. The phenolic content obtained was approximately 200 mg of gallic acid equivalents/100 g tomatoes, and the saponin content was 1000 mg of tomatine equivalents/100 g tomatoes. Total carbohydrate content was different between fresh and frozen tomatoes, 1812 mg of D(+)- glucose equivalents/100 g tomatoes versus 1269 mg/100 g, respectively. In terms of antioxidant activity, a value around 100 mg of Trolox equivalents/100 g of tomatoes was obtained in the 2,2-diphenyl-1-picrylhydrazyl assay, whereas a value of 558 mg of Trolox equivalents/100 mg fresh tomatoes versus 452 mg of Trolox equivalents/100 g frozen tomatoes was obtained by cupric ion reducing antioxidant capacity assay. SWE extraction proved to be a valuable method to extract glycoalkaloids from green tomatoes. The obtained extracts have the potential to be used as ingredients and actives in the cosmetic and pharmaceutical industries.