# Evidence for a model of conformational change by the Plasmodium falciparum circumsporozoite protein during sporozoite development in the mosquito host through the use of camelid single-domain antibodies

## TL;DR

This study shows that the Plasmodium falciparum circumsporozoite protein changes shape during development in mosquitoes, using camelid antibodies to track these changes.

## Contribution

The study provides new evidence for conformational changes in PfCSP during sporozoite development in mosquitoes.

## Key findings

- Camelid sdAbs specifically target PfCSP's αTSR domain without cross-reacting with P. berghei CSP.
- Most sdAbs recognize native PfCSP on the SPZ surface but do not inhibit hepatocyte binding or invasion.
- Structural changes in PfCSP are indicated by altered α-epitope exposure in midgut versus salivary gland SPZs.

## Abstract

Plasmodium sporozoites (SPZs) are formed in the Anopheles mosquito midgut from where they travel to the salivary glands and subsequently to the mammalian liver after deposition into the skin. The SPZ’s main surface antigen, the circumsporozoite protein (CSP), plays a pivotal role in SPZ biology and constitutes the immunodominant target for host antibodies. In this study, we raised single-domain antibodies (sdAbs) against CSP from P. falciparum (PfCSP) by immunizing two alpacas with recombinant versions of the antigen. We found that all identified sdAbs specifically target PfCSP’s globular αTSR domain without cross-reacting with P. berghei CSP. Further characterization revealed that most sdAbs recognize native PfCSP on the SPZ surface, although they do not have any inhibitory effect on hepatocyte binding and invasion. Structural studies showed that all binders target the previously identified α-epitope, confirming the non-protective nature of this epitope. Comparison of sdAb binding to midgut and salivary gland SPZs revealed a shift in the exposure and accessibility of the α-epitope. Hence, our findings provide further evidence that CSP undergoes structural changes during SPZ development in the mosquito host.