# Changes in Serum Proteomic Profiles at Different Stages of Pregnancy Toxemia in Goats

## TL;DR

This study identifies serum protein changes in goats with pregnancy toxemia, offering potential biomarkers for better diagnosis and treatment.

## Contribution

The study identifies novel serum proteomic biomarkers that differentiate subclinical and clinical stages of pregnancy toxemia in goats.

## Key findings

- Twenty-five proteins showed significant differential expression in pregnancy toxemia compared to healthy goats.
- Altered proteins were linked to reactive oxygen species, cytokine activation, and lipid metabolism processes.
- The findings suggest potential for rapid diagnostic tests using serum proteomic biomarkers.

## Abstract

In human medicine, serum proteome profiles are used to differentiate metabolic diseases and evaluate their pathophysiology. Understanding the serum proteome profiles of goats with pregnancy toxemia might help identify the proteomes and pathways responsible for the development of this disease and improve diagnosis and treatment.

Determine biomarkers that differentiate healthy goats from goats with subclinical pregnancy toxemia (SPT) and clinical pregnancy toxemia (CPT) using serum proteome analysis. In addition, serum protein differences were evaluated to distinguish between SPT and CPT.

Thirty‐five hair goats were included, with 15 in the SPT group, 10 in the CPT group, and 10 in the control group.

The cross‐sectional study was conducted with animals from goat farms in Bingol, Türkiye, between December 2023 and May 2024. Serum samples were investigated using mass spectrometry‐based proteomic analysis.

Proteomic profiles showed significant variation in relative protein abundance. Twenty‐five proteins were significantly (p ≤ 0.01) differentially expressed between animals with pregnancy toxemia and those of the control group, with ≥ 2‐fold changes in abundance. Proteins involved in cellular, biological, and molecular processes, including processes related to reactive oxygen species, cytokine activation, acute phase response signaling, lipid metabolism, and antimicrobial activity were altered in animals with pregnancy toxemia.

The proteomic candidates identified are biomarkers that may facilitate the diagnosis, treatment, and management of pregnancy toxemia in goats. Serum proteomic biomarkers could be used in rapid tests in the future and may improve the management of pregnancy toxemia on farms.