# The complete genome sequences of Bacillus velezensis B26: a promising biocontrol agent and biofertilizer

**Authors:** Venkatesh Kamath B, Sandeep Mallya, Subrahmanyam Volety Mallikarjuna, Kiran Kumar Kolathur, Diego Sauka, Surajit Basak, Saranya Nallusamy

PMC · DOI: 10.12688/f1000research.160546.1 · F1000Research · 2025-02-06

## TL;DR

This paper reports the full genome sequence of Bacillus velezensis B26, a beneficial bacterium that helps control plant diseases and promote growth.

## Contribution

The study provides the complete genome sequence of a newly isolated strain of B. velezensis with biocontrol and biofertilizer potential.

## Key findings

- Strain B26 has a 3.95 million base pair genome with 46.3% G+C content.
- The genome includes genes related to stress response, nutrient metabolism, and virulence.
- The genome data are publicly available for further research and application.

## Abstract

Bacillus velezensis is a bacterium widely recognized for its biocontrol properties and ability to promote plant growth. This study presents the whole-genome sequence of
B. velezensis B26, a newly identified strain isolated from chicken carcass soil in Udupi, India. The bacterium showed strong activity against fungal pathogens and exhibited diverse enzymatic activities. The whole-genome sequencing was executed using Illumina technologies. Assembly revealed that strain B26 possesses a genome of 3,946,698-bp with a G+C content of 46.3%. Genome annotation identified 3776 protein-coding genes, 1 rRNA gene, 50 tRNA genes, 5 ncRNA genes, and 59 pseudogenes. Functional analysis of the
B. velezensis B26 genome revealed 216 genes involved in carbohydrate metabolism, 3 genes in potassium metabolism, 148 genes linked for cofactors, vitamins, prosthetic groups and pigments, 10 genes involved in phosphorus metabolism, 24 genes associated with iron acquisition and metabolism, 20 genes for nitrogen metabolism, 6 genes involved in sulfur metabolism, 6 genes in secondary metabolism, 12 genes associated with metabolism of aromatic compounds, 43 genes involved in stress response and 36 genes associated with virulence, disease and defence. The raw sequence data generated in this work have been deposited in the NCBI database and the genome sequence is available under the accession number JAYKOV000000000. These genomic data provide insight into the biocontrol ability and plant-growth promoting capabilities of
B. velezensis B26.

## Full-text entities

- **Diseases:** fungal (MESH:D009181)
- **Chemicals:** sulfur (MESH:D013455), iron (MESH:D007501), potassium (MESH:D011188), nitrogen (MESH:D009584), carbohydrate (MESH:D002241), phosphorus (MESH:D010758)
- **Species:** Gallus gallus (bantam, species) [taxon 9031]

## Full text

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## Figures

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## References

16 references — full list in the complete paper: https://tomesphere.com/paper/PMC12149806/full.md

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Source: https://tomesphere.com/paper/PMC12149806