CK2 regulates somatostatin expression in pancreatic delta cells
Selina Wrublewsky, Annika Clemenz, Anne S. Boewe, Cedric Wilden, Caroline Bickelmann, Claudia Götz, Patrick E. MacDonald, Matthias W. Laschke, Emmanuel Ampofo
TL;DR
This study shows that the CK2 kinase regulates somatostatin expression in pancreatic delta cells, affecting hormone regulation in the endocrine pancreas.
Contribution
The novel finding is that CK2 regulates somatostatin expression in pancreatic delta cells, expanding its role in endocrine hormone regulation.
Findings
CK2 inhibition increases somatostatin gene expression and secretion in RIN14B cells.
CK2 regulates somatostatin expression in isolated murine and human islets under physiological conditions.
CK2 controls the expression of pancreatic endocrine hormones, including somatostatin.
Abstract
Pancreatic and duodenal homeobox protein (PDX)1 is a major transcription factor for the regulation of insulin, glucagon and somatostatin (SST) expression. PDX1 is phosphorylated by CK2 and inhibition of this kinase results in an increased insulin and decreased glucagon secretion. Therefore, we speculated in this study that CK2 also affects SST expression. To test this, we analyzed the effects of the two CK2 inhibitors CX-4945 and SGC as well as of PDX1 overexpression on SST expression and secretion in RIN14B cells by qRT-PCR, luciferase assays, Western blot and ELISA. SST expression and secretion were additionally assessed in isolated murine and human islets exposed to the CK2 inhibitors. Moreover, we determined the expression and secretion of the pancreatic endocrine hormones in CX-4945-treated mice. We found a suppressed SST expression in RIN14B cells due to a methylated SST promoter,…
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Taxonomy
TopicsPancreatic function and diabetes · Neuroendocrine Tumor Research Advances · Pancreatic and Hepatic Oncology Research