# Peimine Promotes Skin Wound Repair in Mice by Activating the Notch1 Signaling Pathway in Fibroblasts

## TL;DR

Peimine speeds up skin wound healing in mice by boosting the Notch1 signaling pathway in fibroblasts, leading to faster tissue repair.

## Contribution

This study reveals that peimine promotes wound healing via Notch1 pathway activation in fibroblasts, offering new therapeutic insights.

## Key findings

- Peimine treatment significantly increased wound closure rates and granulation tissue formation in mice.
- Peimine elevated collagen IIIα1, PCNA, and CD31 protein levels, indicating enhanced tissue regeneration.
- Notch1 signaling pathway components were upregulated in both in vivo and in vitro models following peimine treatment.

## Abstract

This study aimed to investigate the effects of peimine on cutaneous wound healing efficiency and tissue regeneration in murine models, while exploring its regulatory mechanisms through the Notch1 signaling pathway. Full‐thickness circular skin defects (8‐mm diameter) were surgically created on the dorsal surface of C57BL/6 mice, with subsequent daily topical administration of peimine at 1 and 4 mg · kg−1 doses. Parallel in vitro experiments using NIH/3T3 fibroblasts were conducted with peimine treatments at 25 and 100 μmol · L−1 concentrations to assess neurogenic locus notch homolog protein 1 (Notch1) pathway activation. Key findings demonstrated that peimine treatment significantly enhanced both the rate of wound closure and granulation tissue formation. Histological analysis revealed increased epidermal thickness in peimine‐treated groups compared to controls. The compound promoted extracellular matrix remodeling in the dermal layer, evidenced by elevated protein expression of collagen IIIα1 (Col3α1), proliferating cell nuclear antigen (PCNA), and the endothelial marker (CD31). Western blot analysis confirmed consistent upregulation of Notch1 pathway components in both in vivo wound tissues and in vitro fibroblast cultures, indicating that peimine accelerates wound repair through Notch1 signaling activation.

This study clarifies the potential therapeutic effect of peimine on promoting skin wound repair, which might benefit from accelerated granulation tissue formation, epithelial regeneration, collagen deposition, proliferation, and vascular regeneration via regulating the Notch1 signaling pathway on fibroblasts in mice. It provides new insights for the development of peimine and directing its clinical application.