# Aberrant glycosylation patterns as potential biomarkers for diagnosis and disease progression in bullous pemphigoid

## TL;DR

This study identifies abnormal sugar patterns in body fluids of bullous pemphigoid patients that could help diagnose and track the disease.

## Contribution

The study reveals novel glycosylation patterns in serum, blister fluid, and saliva that correlate with disease severity in bullous pemphigoid.

## Key findings

- Increased sialic acid, galactose, and fucose glycosylation in serum proteins of BP patients.
- Enhanced fucosylation and biantennary N-glycan glycosylation in blister fluid proteins.
- Abnormal glycosylation in saliva correlates with disease severity and could serve as non-invasive biomarkers.

## Abstract

Bullous pemphigoid (BP) is a prototypical autoimmune disease characterized by the production of autoantibodies against hemidesmosomal proteins BP180 and BP230. Aberrant glycosylation has emerged as a possible mechanism linked to the pathogenesis and progression of autoimmune diseases. However, the precise alterations in glycosylation in BP remain largely unknown. In this study, we explored the molecular mechanisms of abnormal glycosylation in BP pathogenesis.

We systematically investigated the glycosylation changes in serum, blister fluid, and saliva from BP patients using lectin microarray assays and lectin-based enzyme-linked immunosorbent assays.

Our findings revealed increased glycosylation modifications of sialic acid, galactose, and fucose in serum proteins from BP patients, as well as enhanced fucosylation, galactosylation, and biantennary N-glycan glycosylation in blister fluid proteins. Notably, these abnormal modifications of monosaccharides correlated with the clinical indicators of BP. Furthermore, we observed that glycosylation patterns in saliva were associated with disease severity, suggesting their potential as valuable non-invasive diagnostic markers for BP.

These discoveries indicate that aberrant glycosylation patterns may provide insights into the pathogenesis of BP and serve as potential biomarkers for diagnosing and monitoring the disease.