# Comprehensive biomedicine assessment of Apteranthes tuberculata extracts: Phytochemical analysis and multifaceted pharmacological evaluation in animal models

## TL;DR

This study explores the medicinal potential of Apteranthes tuberculata extract in mice, showing antioxidant, pain-relieving, anti-inflammatory, and gut-regulating effects.

## Contribution

The study provides a comprehensive pharmacological evaluation of Apteranthes tuberculata extract across multiple biological systems in animal models.

## Key findings

- Ethanolic extract of Apteranthes tuberculata showed significant antioxidant activity in vitro.
- The extract exhibited analgesic effects at 200 mg/kg and complex anti-inflammatory responses in mice.
- EEAT influenced body temperature regulation and enhanced gastrointestinal motility at higher doses.

## Abstract

This study comprehensively analyzes the pharmacological effects of ethanolic extract of Apteranthes tuberculata (EEAT) on various physiological parameters in mice.

The research aimed to quantify flavonoid and phenol contents across different extraction methods, with a focus on the superior efficacy of the ethanolic extract.

In vitro assays were conducted to assess the antioxidant activity of EEAT, revealing dose-dependent effects and significant inhibition percentages.

EEAT exhibited notable analgesic effects in the writhing response test, particularly at 200 mg/kg, indicating its potential as a natural analgesic. Additionally, its anti-inflammatory effects were complex and dose-dependent in a carrageenan-induced paw edema model. The extract also showed significant changes in body temperature regulation following Brewer’s yeast-induced fever, revealing a distinct pattern of initial hypothermia followed by gradual re-elevation. Furthermore, EEAT demonstrated regulatory effects on gastrointestinal motility, with higher doses enhancing intestinal transit in charcoal meal tests.

This study highlights the pharmacological potential of EEAT as a natural therapeutic agent for antioxidant, analgesic, anti-inflammatory, and gastrointestinal regulation, warranting further investigation into its mechanisms of action and therapeutic applications.