# Expressing intrinsically-disordered tardigrade proteins has positive effects on acute but not chronic stress tolerance in Saccharomyces cerevisiae

## TL;DR

Tardigrade proteins help yeast survive short-term stress but not long-term stress, offering potential for industrial bioprocesses.

## Contribution

The study shows tardigrade proteins can enhance acute stress tolerance in yeast without major growth issues.

## Key findings

- MAHS and RvLEAM improve acute hyperosmotic stress tolerance in yeast.
- CAHS3 and RvLEAM enhance desiccation survival in yeast.
- Tardigrade proteins do not improve chronic stress tolerance in yeast.

## Abstract

The production of high value and commodity chemicals, biopharmaceuticals and biofuels using Saccharomyces cerevisiae is hindered by various stress factors that affect yield and efficiency. Tardigrades, known for their remarkable stress tolerance, express unique proteins responsible for their resilience. This study evaluates the impact of expressing the tardigrade proteins CAHS3, MAHS, and RvLEAM on stress tolerance in S. cerevisiae. Our results show that high yields of these proteins do not impede yeast growth, except for CAHS3, which reduces proliferation. Expression of MAHS enhances acute heat tolerance, while MAHS and RvLEAM confer increased tolerance to acute hyperosmotic stress. Both CAHS3 and RvLEAM improve desiccation survival. However, these proteins do not provide benefits under chronic stress conditions such as prolonged exposure to high temperature, hyperosmotic stress, or solvents. These findings highlight the potential utility of tardigrade proteins for transient stress protection in industrial bioprocesses and suggest future engineering approaches for improved stress tolerance in yeast.