# Automated tissue dissociation for the establishment of human intestinal organoids

## TL;DR

This paper introduces a semi-automated method for efficiently and consistently creating human intestinal organoids from stem cells.

## Contribution

A semi-automated mechanical tissue dissociation technique is introduced for standardized and efficient HIO production.

## Key findings

- The semi-automated method improves workflow efficiency and standardization.
- The technique maintains high organoid viability consistently.
- It streamlines the dissociation and culture of intestinal LGR5+ stem cells.

## Abstract

Human intestinal Organoids (HIOs) may be set up from primary human tissue following dissociation, release, and culture of intestinal LGR5 + stem cells. Here, we characterise the use of a semi-automated mechanical tissue dissociation technique, which demonstrates an expedited workflow and streamlined protocol, standardising key aspects of the process while consistently maintaining a high level of cultured organoid viability.

The online version contains supplementary material available at 10.1038/s41598-025-03905-9.