# Open-source software utilization for zebrafish embryos behavior test

## TL;DR

This paper introduces an accessible and cost-effective method for analyzing zebrafish embryo behavior using open-source software.

## Contribution

The paper provides a practical, open-source alternative to expensive commercial tools for behavioral analysis.

## Key findings

- The method uses ImageJ and VirtualDub to convert video formats and measure locomotive activity.
- The approach is effective for generating locomotive parameters relevant to neurological research.
- The technique is adaptable for use in other model systems.

## Abstract

This work described simple methods for measuring locomotive activity using open-source software, ImageJ1.54fFiji, and VirtualDub2. The significance of animal behavior is a mirror of brain activity, which can give information implicated with neurological diseases. Commercial behavioral analysis software frequently needs expertise and expenses high costs due to equip a specific instrument to use of software, thereby encouraging a trend toward open-source alternatives that are both accessible and effective. Here, we explained how to convert video format, measure movement, and produce useful locomotive parameters to aid in the assessment of zebrafish embryos. This method could be easily translated for use in other model systems. This methodology seeks to streamline behavioral quantification in research contexts, encouraging broader research aspects.