# Validation and forensic application of a new 36 X-chromosomal short tandem repeat loci multiplex system

**Authors:** Kaiqin Chen, Ruiyang Tao, Yiling Qu, Junnan Lu, Yuan Ping, Yilun Zhang, Pengyu Chen, Chengtao Li

PMC · DOI: 10.1093/fsr/owae029 · Forensic Sciences Research · 2024-04-23

## Abstract

X-chromosomal short tandem repeats are indispensable in specific cases, distinct from autosomal and Y chromosome genetic markers. SureID® X37 is an innovative six-colour fluorescence multiplex detection system that can simultaneously amplify 36 X-chromosomal short tandem repeat loci (including DXS6795, DXS7132, DXS8378, DXS10101, DXS10103, DXS10079, DXS10134, GATA165B12, GATA172D05, HPRTB, DXS6810, DXS10135, DXS6797, DXS10074, DXS7424, DXS9902, DXS7423, DXS10148, DXS10162, DXS6809, DXS10159, GATA31E08, DXS6803, DXS10075, DXS6807, DXS10164, DXS6789, DXS10146, DXS7133, DXS6804, DXS981, DXS9895, DXS101, DXS6800, DXS9907, and DXS8377) and the Amelogenin locus. In this study, we validated its suitability for forensic identification per Scientific Working Group on DNA Analysis Methods guidelines, including PCR conditions, precision, accuracy, case-type samples, sensitivity, repeatability, reproducibility, species specificity, stability, stutter calculation, and DNA mixtures. Additionally, 577 Chinese Han individuals were used to investigate the utility of the system in forensic population genetics. The results indicated that the system is sensitive, stable, and reliable and is suitable for typical forensic cases. Subsequent population investigations confirmed that it serves as a potent supplementary tool in forensic applications.

## Full-text entities

- **Diseases:** PD (MESH:D010468), gang rape (MESH:C537457), Stutter (MESH:D013342)
- **Chemicals:** melanin (MESH:D008543), water (MESH:D014867), heme (MESH:D006418), EDTA (MESH:D004492), humic acid (MESH:D006812), DXS10135 (-), gold (MESH:D006046), carbamide (MESH:D014508), indigotin (MESH:D007203), metal (MESH:D008670), formamide (MESH:C031066)
- **Species:** Gallus gallus (bantam, species) [taxon 9031], Sus scrofa (pig, species) [taxon 9823], Anser sp. (goose, species) [taxon 8847], Homo sapiens (human, species) [taxon 9606], Oryctolagus cuniculus (domestic rabbit, species) [taxon 9986], Canis lupus familiaris (dog, subspecies) [taxon 9615], Macaca (macaque, genus) [taxon 9539], Rattus norvegicus (brown rat, species) [taxon 10116], Bos taurus (bovine, species) [taxon 9913], Ovis aries (domestic sheep, species) [taxon 9940]

## Full text

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## Figures

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## References

53 references — full list in the complete paper: https://tomesphere.com/paper/PMC12140019/full.md

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Source: https://tomesphere.com/paper/PMC12140019